Movie S1: Glk1-GFP Polymerization is Induced by Glucose. Glk1-GFP cells grown to saturation were loaded into a flow cell. At the start of the movie, CSM-Glucose medium is flowed over the cells, inducing Glk1 polymerization. The movie is a maximum intensity projection of a confocal z-stack. Images were taken at 1 minute intervals.

Movie S2: Glk1-GFP Depolymerizes When Glucose is Removed. Glk1-GFP cells grown to saturation were loaded into a flow cell and washed into glucose containing medium. At the start of the movie, glucose is washed out by washing the cells into CSM medium with no carbon source. The movie is a single confocal slice with continuous capture. Images were taken at 100ms intervals.