Movie S1:
Glk1-GFP Polymerization is Induced by Glucose. Glk1-GFP
cells grown to saturation were loaded into a flow cell. At the start of the
movie, CSM-Glucose medium is flowed over the cells, inducing Glk1
polymerization. The
movie is a maximum intensity projection of a confocal z-stack. Images were
taken at 1 minute intervals.
Movie S2: Glk1-GFP
Depolymerizes When Glucose is Removed. Glk1-GFP cells grown to saturation were
loaded into a flow cell and washed into glucose containing medium. At the start
of the movie, glucose is washed out by washing the cells into CSM medium with
no carbon source. The
movie is a single confocal slice with continuous capture. Images were taken at
100ms intervals.